Elimination of Solanum nigrum ilarvirus 1 and Apple Hammerhead Viroid from Apple Cultivars Using Antivirals Ribavirin, Rimantadine, and Zidovudine

Apple hammerhead viroid (AHVd) was detected in the apple cultivar ‘Šampion’ and mixed infection with Solanum nigrum ilarvirus 1 (SnIV-1) cultivars ‘Selena’ ‘Jonagored Supra’, using a high-throughput sequencing method. Experiments were conducted to eliminate both pathogens apples meristem tip cultures combination antivirotics ribavirin, rimantadine, zidovudine. Elimination of verified by repeated RT-PCR qRT-PCR assays after 7–11 months. SnIV-1 from all successful each three at concentrations 20, 40, 80 mg L−1. AHVd also achieved, although less effectively only ribavirin concentration range 20–160